Recombinant Mouse Spn Protein, Myc/DDK-tagged

• Cat.No.: Spn-6102M
• Product Overview: Purified recombinant protein of mouse full-length sialophorin (Spn), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 40.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Spn sialophorin [ Mus musculus (house mouse) ]
• Official Symbol: Spn
• Synonyms: SPN; sialophorin; leukosialin; 3E8 antigen; lymphocyte antigen 48; leukocyte sialoglycoprotein; B-cell differentiation antigen LP-3; Cd43; Ly48; Galgp; Ly-48; A630014B01Rik
• Gene ID: 20737
• mRNA Refseq: NM_001037810
• Protein Refseq: NP_001032899
• UniProt ID: P15702