Recombinant Mouse Smad5 protein(Thr2-Ser465), His&GST-tagged

• Cat.No.: Smad5-3245M
• Product Overview: Recombinant Mouse SMAD5 (P97454) (Thr2-Ser465) was expressed in Insect Cells with a N-terminal polyhistidine tag followed by a GST tags.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Thr2-Ser465
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse SMAD5/GST chimera consists of 701 amino acids and has a calculated molecular mass of 79.9 kDa. The recombinant protein migrates as an approximately 75-88 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Smad5 MAD homolog 5 (Drosophila) [ Mus musculus ]
• Official Symbol: Smad5
• Synonyms: SMAD5; MAD homolog 5 (Drosophila); mothers against decapentaplegic homolog 5; Smad 5; mSmad5; dwarfin-C; SMAD family member 5; mothers against DPP homolog 5; Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
• Gene ID: 17129
• mRNA Refseq: NM_001164041
• Protein Refseq: NP_001157513