Recombinant Mouse Ska1 Protein, Myc/DDK-tagged

• Cat.No.: Ska1-5891M
• Product Overview: Purified recombinant protein of mouse full-length spindle and kinetochore associated complex subunit 1 (Ska1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 29.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ska1 spindle and kinetochore associated complex subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ska1
• Synonyms: SKA1; spindle and kinetochore associated complex subunit 1; spindle and kinetochore-associated protein 1; AV117428; 2810433K01Rik
• Gene ID: 66468
• mRNA Refseq: NM_001164355
• Protein Refseq: NP_001157827
• UniProt ID: Q9CPV1