Recombinant Mouse Shtn1 Protein, Myc/DDK-tagged

• Cat.No.: Shtn1-5872M
• Product Overview: Purified recombinant protein of mouse full-length shootin 1 (Shtn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth. Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Plays also a role in regenerative neurite outgrowth. In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons.
• Molecular Mass: 71.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Shtn1 shootin 1 [ Mus musculus (house mouse) ]
• Official Symbol: Shtn1
• Synonyms: Shtn1; shootin 1; sho; Shootin1; mKIAA1598; 4930506M07Rik; shootin-1
• Gene ID: 71653
• mRNA Refseq: NM_001114312
• Protein Refseq: NP_001107784
• UniProt ID: Q8K2Q9