Recombinant Mouse Selectin, Endothelial Cell, Fc-His

• Cat.No.: Sele-1720M
• Product Overview: Recombinant Mouse E Selectin (Trp22 -Pro557) produced inNSO Cellswas fused to Fc region of human IgG1 (Pro100 - Lys330) - 6xHis tag

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc&His
• Protein Length: 22-557 a.a.
• Description: E-selectin, also known as CD62E is an inducible leukocyte adhesion glycoprotein specifically expressed by endothelial cells. E-selectin helps initiate recruitment of circulating leukocytes to cutaneous, bone and inflamed tissues. E-selectin is found in inflammatory skin lesions in psoriasis, contact dermatitis, and delayed- type hypersensitivity, in arthritic joints, and in heart and renal allografts undergoing rejection. E-selectin-targeting antibody drug conjugate has potential as a prostate cancer therapy.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse E-Selectin is Mr 85.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 150 kDa.
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse E Selectin coated plates (2 μg/ml with 100 μl/well), approximately 85 - 100% will adhere.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: Sele selectin, endothelial cell [ Mus musculus ]
• Synonyms: Sele; selectin, endothelial cell; Elam; CD62E; E-selectin
• Gene ID: 20339
• mRNA Refseq: NM_011345
• Protein Refseq: NP_035475
• UniProt ID: Q00690
• Chromosome Location: 1 H2.2; 1 86.6 cM
• Pathway: Cell adhesion molecules (CAMs)
• Function: Binding; protein binding; sugar binding