Recombinant Mouse Sele protein(Met1-Pro564), His-tagged

• Cat.No.: Sele-8680M
• Product Overview: Recombinant Mouse SELE (NP_035475.1) (Met1-Pro564) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-564 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse E-Selectin coated plates (2 μg/mL with 100 μL/well), approximately 30%-60% cells will adhere for 1 hour incubation at 37°C.
• Molecular Mass: The recombinant mouse SELE comprises 547 amino acids and has a predicted molecular mass of 60 kDa. The apparent molecular mass of the protein is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sele selectin, endothelial cell [ Mus musculus ]
• Official Symbol: Sele
• Synonyms: SELE; selectin, endothelial cell; E-selectin; ELAM-1; LECAM2; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2; Elam; CD62E
• Gene ID: 20339
• mRNA Refseq: NM_011345
• Protein Refseq: NP_035475