Recombinant Mouse Rnf7 Protein, Myc/DDK-tagged

• Cat.No.: Rnf7-5560M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 7 (Rnf7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 12.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnf7 ring finger protein 7 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf7
• Synonyms: RNF7; ring finger protein 7; RING-box protein 2; sensitive to apoptosis gene protein; SAG; Rbx2
• Gene ID: 19823
• mRNA Refseq: NM_011279
• Protein Refseq: NP_035409
• UniProt ID: Q9WTZ1