Recombinant Mouse Reticulon 4 Receptor, His-tagged

• Cat.No.: Rtn4r-4009M
• Product Overview: A DNA sequence encoding the the mature form of mouse RTN4R (NP_075358.1) (Met 1-Ser 447) was fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 1-447 a.a.
• Description: Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer"s disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.
• Form: Lyophilized from sterile PBS, pH7.4
• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70μ
• Predicted N terminal: Cys 27
• Molecular Mass: The recombinant mouse RTN4R/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 669 amino acids and has a predicted molecular mass of 73.8 kDa. Due to glycosylation, rmRTN4R/Fc monomer migrates as an approximately 95-100 kDa band in SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Rtn4r reticulon 4 receptor [ Mus musculus ]
• Official Symbol: Rtn4r
• Synonyms: Rtn4r; reticulon 4 receptor; NgR; NOGOR; reticulon-4 receptor; nogo receptor; Nogo-66 receptor
• Gene ID: 65079
• mRNA Refseq: NM_022982
• Protein Refseq: NP_075358
• UniProt ID: Q99PI8
• Chromosome Location: 16 B1
• Function: protein binding; receptor activity