Recombinant Mouse Resistin Like Alpha, Flag-tagged

• Cat.No.: Retnla-4156M
• Product Overview: RELM-alpha Mouse Recombinant is manufactured with a signal sequence of phage fd (20aa) and C-terminal fusion of flagTag (10aa). The RELM-alpha Flag-Tagged Fusion Protein is a 13.3 kDa protein containing 91 amino acid residues with 30 additional amino acid residues - signal sequence of phage fd, flagTag (underlined). MKKLLFAIPL VVPFYSHSTM VNTDETIEII VENKVKELLA NPANYPSTVT TLSCTSVKT MNRWASCPAG MTATGCACGF ACGSWEIQSG DTCNCLCLLV DWTTARCCQL SLEDYKDDDD K.

Specification

• Species: Mouse
• Source: Mouse
• Tag: Flag
• Description: Bronchoalveolar lavage fluid from mice with experimentally induced allergic pulmonary inflammation contains a novel 9.4 kDa cysteine-rich secreted protein, RELM-alpha (FIZZ1, found in inflammatory zone). RELM-alpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue stroma. Murine RELM-alpha (FIZZ1) is the founding member of a new gene family including two other murine genes expressed, respectively, in intestinal crypt epithelium (RELM-beta) and white adipose tissue (Resistin), and two related human genes.
• Form: Sterile filtered and lyophilized from 0.5 mg/ml in 5mM Tris pH 7.5, 25mM NaCl.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Specificity: The amino acid sequence of the RELM-alpha Mouse recombinant is 100% homologous to the amino acid sequence of the RELM-alpha Mouse.
• Purification Method: Two-step procedure using size exclusion chromatography before and after refolding.
• Applications: Western blotting, ELISA
• Physical Appearance: Sterile filtered colorless solution.
• Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Gene Information

• Gene Name: Retnla resistin like alpha [ Mus musculus ]
• Official Symbol: Retnla
• Synonyms: Retnla; resistin-like alpha; HIMF; Xcp2; Fizz1; RELMa; Fizz-1; RELMalpha; RELM-alpha; 1810019L16Rik; found in inflammatory zone 1; hypoxia-induced mitogenic factor; cysteine-rich secreted protein FIZZ1; cysteine-rich secreted protein A12-gamma; parasite-induced macrophage novel gene 1 protein
• Gene ID: 57262
• mRNA Refseq: NM_020509
• Protein Refseq: NP_065255
• UniProt ID: Q9EP95
• Chromosome Location: 16 B5; 16 32.6 cM
• Function: hormone activity