Recombinant Mouse Rab5a Protein, Myc/DDK-tagged

• Cat.No.: Rab5a-5326M
• Product Overview: Purified recombinant protein of mouse full-length RAB5A, member RAS oncogene family (Rab5a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
• Molecular Mass: 23.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rab5a RAB5A, member RAS oncogene family [ Mus musculus (house mouse) ]
• Official Symbol: Rab5a
• Synonyms: RAB5A; RAB5A, member RAS oncogene family; ras-related protein Rab-5A; AI663973; AU021172; 2410015H04Rik
• Gene ID: 271457
• mRNA Refseq: NM_025887
• Protein Refseq: NP_080163
• UniProt ID: Q9CQD1