Recombinant Mouse PVRIG protein, hFc-Avi-tagged, Biotinylated

• Cat.No.: PVRIG-1641M
• Product Overview: Biotinylated Recombinant Mouse PVRIG protein(A0A1B0GS01)(Ser35-Asp165), fused with C-terminal hFc and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ser35-Asp165
• Tag: C-hFc-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse Nectin-2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.