Recombinant Mouse Psme4 Protein, MYC/DDK-tagged

• Cat.No.: Psme4-229M
• Product Overview: Purified recombinant protein of full-length mouse proteasome (prosome, macropain) activator subunit 4 (Psme4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 211.6 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Psme4 proteasome (prosome, macropain) activator subunit 4 [ Mus musculus (house mouse) ]
• Official Symbol: Psme4
• Synonyms: Psme4; proteasome (prosome, macropain) activator subunit 4; TEMO; AA409398; AU041366; mKIAA0077; proteasome activator complex subunit 4; proteasome activator PA200
• Gene ID: 103554
• mRNA Refseq: NM_134013
• Protein Refseq: NP_598774
• UniProt ID: Q5SSW2