Recombinant Mouse Proc protein, His & GST-tagged

• Cat.No.: Proc-826M
• Product Overview: Recombinant Mouse Proc aa. (Lys197~Lys436 (Accession # P33587)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes the vitamin K-dependent protein C, which plays a vital role in the anticoagulation pathway. The encoded protein undergoes proteolytic processing including activation by thrombin-thrombomodulin complex to form the anticoagulant serine protease that degrades activated coagulation factors. A complete lack of the encoded protein in mice results in severe perinatal consumptive coagulopathy in the brain and liver, resulting in death within 24 hours after birth. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein.
• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 50&80kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Lys197~Lys436
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: Proc protein C [ Mus musculus (house mouse) ]
• Official Symbol: Proc
• Synonyms: PC; anticoagulant protein C; autoprothrombin IIA; blood coagulation factor XIV; inactivator of coagulation factors Va, VIII; vitamin K-dependent protein C
• Gene ID: 19123
• mRNA Refseq: NM_001042767.3
• Protein Refseq: NP_001036232.1
• UniProt ID: P33587