Recombinant Mouse Pomc protein, His-tagged

• Cat.No.: Pomc-6794M
• Product Overview: Recombinant Mouse Pomc aa. (Trp27~Gln235 (Accession # P01193)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Trp27~Gln235
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Pomc pro-opiomelanocortin-alpha [ Mus musculus (house mouse) ]
• Official Symbol: Pomc
• Synonyms: BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH; adrenal corticotropic hormone; adrenocorticotropic hormone; alpha-melanocyte stimulating hormone; beta-endorphin; corticotropin-like intermediary peptide; corticotropin-lipotropin; melanotropin gamma; met-enkephalin; pro-opiomelanocortin
• Gene ID: 18976
• mRNA Refseq: NM_001278581.1
• Protein Refseq: NP_001265510.1
• UniProt ID: P01193