Recombinant Mouse Polh Protein, Myc/DDK-tagged

• Cat.No.: Polh-4989M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (DNA directed), eta (RAD 30 related) (Polh), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine. Particularly important for the repair of UV-induced pyrimidine dimers Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 76.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Polh polymerase (DNA directed), eta (RAD 30 related) [ Mus musculus (house mouse) ]
• Official Symbol: Polh
• Synonyms: POLH; polymerase (DNA directed), eta (RAD 30 related); DNA polymerase eta; RAD30 homolog A; DNA-directed DNA polymerase eta; xeroderma pigmentosum variant type protein homolog; XPV; RAD30A
• Gene ID: 80905
• mRNA Refseq: NM_030715
• Protein Refseq: NP_109640
• UniProt ID: Q9JJN0