Recombinant Mouse Pgk1 Protein, Myc/DDK-tagged

• Cat.No.: Pgk1-4819M
• Product Overview: Purified recombinant protein of mouse full-length phosphoglycerate kinase 1 (Pgk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome.
• Molecular Mass: 44.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pgk1 phosphoglycerate kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pgk1
• Synonyms: PGK1; phosphoglycerate kinase 1; Pgk-1; MGC118097
• Gene ID: 18655
• mRNA Refseq: NM_008828
• Protein Refseq: NP_032854
• UniProt ID: P09411