Recombinant Mouse Pgk1 Protein, His-tagged

• Cat.No.: Pgk1-7302M
• Product Overview: Recombinant mouse Pgk1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 1-4170
• Description: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome.
• Form: Liquid
• Molecular Mass: 47.1 kDa
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSHMSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKVKAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFARGTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV
• Purity: > 90 % by SDS-PAGE
• Stability: Shelf life: one year from despatch.
• Storage: Store undiluted at 2-8 centigrade for one week or (in aliquots) at -20 to -80 centigrade for longer. Avoid repeated freezing and thawing.
• Concentration: 1.0 mg/mL (determined by bradford assay)
• Storage Buffer: In Phosphate buffered saline (pH7.4) containing 10 % glycerol, 1 mM DTT.

Gene Information

• Gene Name: Pgk1 phosphoglycerate kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pgk1
• Synonyms: Pgk1; phosphoglycerate kinase 1; Pgk-; Pgk-1; phosphoglycerate kinase 1; EC 2.7.2.3
• Gene ID: 18655
• mRNA Refseq: NM_008828
• Protein Refseq: NP_032854
• UniProt ID: P09411