Recombinant Mouse Pecam1 Protein, Myc/DDK-tagged

• Cat.No.: Pecam1-4788M
• Product Overview: Purified recombinant protein of mouse full-length platelet/endothelial cell adhesion molecule 1 (Pecam1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis
• Molecular Mass: 77.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pecam1 platelet/endothelial cell adhesion molecule 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pecam1
• Synonyms: PECAM1; platelet/endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; PECAM-1/CD31; Cd31; Pecam; C85791; PECAM-1; MGC102160
• Gene ID: 18613
• mRNA Refseq: NM_001032378
• Protein Refseq: NP_001027550
• UniProt ID: Q08481