Recombinant Mouse P4hb protein, His & GST-tagged

• Cat.No.: P4hb-1450M
• Product Overview: Recombinant Mouse P4hb aa. (Asp212~Arg463 (Accession # P09103)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 60.8kDa.
• Protein length: Asp212~Arg463
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: P4hb prolyl 4-hydroxylase, beta polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: P4hb
• Synonyms: PDI; Thbp; ERp59; Pdia1; ER protein 59; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59; p55; prolyl 4-hydroxylase subunit beta; protein disulfide-isomerase
• Gene ID: 18453
• mRNA Refseq: NM_011032.2
• Protein Refseq: NP_035162.1
• UniProt ID: P09103