Recombinant Mouse Optn Protein, Myc/DDK-tagged

• Cat.No.: Optn-4598M
• Product Overview: Purified recombinant protein of mouse full-length optineurin (Optn), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 67 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Optn optineurin [ Mus musculus (house mouse) ]
• Official Symbol: Optn
• Synonyms: OPTN; optineurin; TFIIIA-INTP; NRP; FIP2; HYPL; 4930441O07Rik
• Gene ID: 71648
• mRNA Refseq: NM_181848
• Protein Refseq: NP_862896
• UniProt ID: Q8K3K8