Recombinant Mouse Nudt7 Protein, Myc/DDK-tagged

• Cat.No.: Nudt7-4545M
• Product Overview: Purified recombinant protein of mouse full-length nudix (nucleoside diphosphate linked moiety X)-type motif 7 (Nudt7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Coenzyme A diphosphatase which mediates the cleavage of CoA, CoA esters and oxidized CoA with similar efficiencies, yielding 3',5'-ADP and the corresponding 4'-phosphopantetheine derivative as products. Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs. CoA into 3',5'-ADP and 4'-phosphopantetheine. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
• Molecular Mass: 26.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nudt7 nudix (nucleoside diphosphate linked moiety X)-type motif 7 [ Mus musculus (house mouse) ]
• Official Symbol: Nudt7
• Synonyms: NUDT7; nudix (nucleoside diphosphate linked moiety X)-type motif 7; peroxisomal coenzyme A diphosphatase NUDT7; nudix motif 7; coenzyme A diphosphatase; nucleoside diphosphate-linked moiety X motif 7; 1300007B24Rik; 2210404C19Rik
• Gene ID: 67528
• mRNA Refseq: NM_024437
• Protein Refseq: NP_077757
• UniProt ID: Q99P30