Recombinant Mouse Nt5e protein, His-tagged

• Cat.No.: Nt5e-6720M
• Product Overview: Recombinant Mouse Nt5e aa. (Trp29~phe550 (Accession # Q61503)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Trp29~phe550
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Nt5e 5' nucleotidase, ecto [ Mus musculus (house mouse) ]
• Official Symbol: Nt5e
• Synonyms: NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik; ecto-5'-nucleotidase; 5'-nucleotidase
• Gene ID: 23959
• mRNA Refseq: NM_011851.4
• Protein Refseq: NP_035981.1
• UniProt ID: Q61503