Recombinant Mouse Nek9 Protein, Myc/DDK-tagged

• Cat.No.: Nek9-4370M
• Product Overview: Purified recombinant protein of mouse full-length NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
• Molecular Mass: 107.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nek9 NIMA (never in mitosis gene a)-related expressed kinase 9 [ Mus musculus (house mouse) ]
• Official Symbol: Nek9
• Synonyms: NEK9; NIMA (never in mitosis gene a)-related expressed kinase 9; serine/threonine-protein kinase Nek9; nercc1 kinase; nimA-related protein kinase 9; never in mitosis A-related kinase 9; C130021H08Rik
• Gene ID: 217718
• mRNA Refseq: NM_145138
• Protein Refseq: NP_660120
• UniProt ID: Q8K1R7