Recombinant Mouse Neil1 Protein, Myc/DDK-tagged

• Cat.No.: Neil1-4365M
• Product Overview: Purified recombinant protein of mouse full-length nei endonuclease VIII-like 1 (E. coli) (cDNA clone MGC:49102 IMAGE:5320651), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 28.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Neil1 nei endonuclease VIII-like 1 (E. coli) [ Mus musculus (house mouse) ]
• Official Symbol: Neil1
• Synonyms: Neil1; nei endonuclease VIII-like 1 (E. coli); NEH1; Nei1; 2810450N13Rik; endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; endonuclease VIII; endonuclease VIII-like 1; nei homolog 1; nei-like protein 1; EC 4.2.99.18
• Gene ID: 72774
• mRNA Refseq: NM_028347
• Protein Refseq: NP_082623
• UniProt ID: Q8K4Q6