Recombinant Mouse Nampt Protein, His-tagged

• Cat.No.: Nampt-7402M
• Product Overview: Recombinant mouse visfatin, fused to His-tag, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMNAAAEAEFNMGSSHHHHHHSSGLVPRGSHMNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAPH
• Purity: ≥ 90 % pure by SDS-PAGE
• Stability: Shelf life: one year from despatch.
• Storage: Store (in aliquots) at -20 centigrade. Avoid repeated freezing and thawing.
• Concentration: 1.0 mg/mL
• Storage Buffer: PBS, pH 7.4

Gene Information

• Gene Name: Nampt nicotinamide phosphoribosyltransferase [ Mus musculus (house mouse) ]
• Official Symbol: Nampt
• Synonyms: Nampt; nicotinamide phosphoribosyltransferase; Vi; Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik; nicotinamide phosphoribosyltransferase; pre-B-cell colony-enhancing factor 1 homolog; EC 2.4.2.12
• Gene ID: 59027
• mRNA Refseq: NM_021524
• Protein Refseq: NP_067499
• UniProt ID: Q99KQ4