Recombinant Mouse Nagpa Protein, Myc/DDK-tagged

• Cat.No.: Nagpa-4284M
• Product Overview: Purified recombinant protein of mouse full-length N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (Nagpa), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 56.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nagpa N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase [ Mus musculus (house mouse) ]
• Official Symbol: Nagpa
• Synonyms: Nagpa; N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase; UCE; AI596180; N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase; alpha-GlcNAcase; mannose 6-phosphate-uncovering enzyme; phosphodiester alpha-GlcNAcas; EC 3.1.4.45
• Gene ID: 27426
• mRNA Refseq: NM_013796
• Protein Refseq: NP_038824
• UniProt ID: Q8BJ48