Recombinant Mouse Met Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Met-4062MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Met (NP_032617.2) extracellular domain (Met 1-Asn 929), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant mouse Met is a heterodimer composed of the proteolytically cleaved α and β subunits. The α and β heterodimer consists of 916 amino acids and has a predicted molecular mass of 102 (α =32 + β=70) kDa. The apparent molecular mass of the rmMET heterodimer thus is approximately 43 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: 916
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Met met proto-oncogene [ Mus musculus ]
• Official Symbol: Met
• Gene ID: 17295
• mRNA Refseq: NM_008591
• Protein Refseq: NP_032617