Recombinant Mouse Mecr Protein, Myc/DDK-tagged

• Cat.No.: Mecr-4011M
• Product Overview: Purified recombinant protein of mouse full-length mitochondrial trans-2-enoyl-CoA reductase (Mecr), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates. May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells.
• Molecular Mass: 40.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mecr mitochondrial trans-2-enoyl-CoA reductase [ Mus musculus (house mouse) ]
• Official Symbol: Mecr
• Synonyms: MECR; mitochondrial trans-2-enoyl-CoA reductase; trans-2-enoyl-CoA reductase, mitochondrial; nuclear receptor binding factor 1; Nrbf1; AI195831
• Gene ID: 26922
• mRNA Refseq: NM_025297
• Protein Refseq: NP_079573
• UniProt ID: Q9DCS3