Recombinant Mouse Lrwd1 Protein, Myc/DDK-tagged

• Cat.No.: Lrwd1-3849M
• Product Overview: Purified recombinant protein of mouse full-length leucine-rich repeats and WD repeat domain containing 1 (Lrwd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
• Molecular Mass: 71.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Lrwd1 leucine-rich repeats and WD repeat domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Lrwd1
• Synonyms: LRWD1; leucine-rich repeats and WD repeat domain containing 1; leucine-rich repeat and WD repeat-containing protein 1; ORC-associated protein; origin recognition complex-associated protein; Orca; AU042569; AW548074; 1200011O22Rik
• Gene ID: 71735
• mRNA Refseq: NM_027891
• Protein Refseq: NP_082167
• UniProt ID: Q8BUI3