Recombinant Mouse Lrrc32 & Tgfb1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: Lrrc32 & Tgfb1-6332M
• Product Overview: Biotinylated Recombinant Mouse Lrrc32 & Tgfb1 protein(G3XA59(GARP)&P04202(Latent TGF beta 1))(Ala18-Leu628(LRRC32) & Leu30-Ser390(TGFB1)), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: N-His-Avi
• ProteinLength: Ala18-Leu628(LRRC32) & Leu30-Ser390(TGFB1)
• Tag: N-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 66.6 kDa (GARP)&44.2 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-76 kDa (GARP)&13 kDa&45-47 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• UniProt ID-Weblink: https://www.uniprot.org/uniprot/
• GeneID 2: 21803

Gene Information

• Gene Name: Lrrc32 leucine rich repeat containing 32 [ Mus musculus ]
• Official Symbol: Lrrc32
• Synonyms: LRRC32; leucine rich repeat containing 32; glycoprotein A repetitions predominant; Garp; AI426318; EG434215; D11S833Eh; D7H11S833E
• Gene ID: 434215
• mRNA Refseq: NM_001113379
• Protein Refseq: NP_001106850
• Gene Name 2: Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
• Official Symbol 2: Tgfb1
• Synonyms 2: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta 1; TGF-beta-1; regulatory protein; transforming growth factor-beta 1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• mRNA Refseq 2: NM_011577
• Protein Refseq 2: NP_035707