Recombinant Mouse Lipase, Hepatic, His-tagged

• Cat.No.: Lipc-391M
• Product Overview: Recombinant Mouse LIPC was expressed inE.Coliusing an N-terminal His tag. MW=26 kDa.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Hepatic lipase, also known as LIPC, is a lipolytic enzyme synthesized and secreted by hepatocytes that bind extracellularly to the parenchymal and endothelial cell surfaces in the space of Disse. Clinical studies have shown that an inverse relationship exists between the LIPC activity in postheparin plasma and plasma HDL-C concentrations, suggesting that it can serve as a marker for cardiovascular disease risk and may serve as a target for therapy. The doublet observed in the SDS-PAGE is likely due to incomplete removal of the signal sequence.
• Sequence: 91 - 510.
• Purity: ~95%.
• Specific Activity: n/a.
• Applications: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
• Buffer: 1X PBS containing 0.1% SDS.
• Storage: Store at -70?C. As with any protein, exposing LIPC recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• SDS-PAGE: SDS-PAGE analysis of recombinant LIPC on Coomassie Blue-stained 12% SDS-PAGE gel.

Gene Information

• Gene Name: Lipc lipase, hepatic [ Mus musculus ]
• Synonyms: Lipc; lipase, hepatic; HL; Hpl; AI256194; lipase C; hepatic lipase; EC 3.1.1.3
• Gene ID: 15450
• mRNA Refseq: NM_008280
• Protein Refseq: NP_032306
• UniProt ID: P27656
• Chromosome Location: 9 D; 9 39.0 cM
• Pathway: Glycerolipid metabolism; Metabolic pathways
• Function: catalytic activity; heparin binding; hydrolase activity; triglyceride lipase activity; lipase activity