Recombinant Mouse Leptin Antagonist Triple Mutant

• Cat.No.: Lep-261M
• Product Overview: Leptin Antagonist Triple Mutant Mouse Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, LEP was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Mouse Recombinant was purified by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: Mouse
• Tag: Non
• Form: The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Purity: Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Physical Appearance: White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln Biological ActivityProSpecμs Leptin Antagonist Triple Mutant Mouse Recombinant is capable of inhibiting Leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays.
• Protein content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Storage: Lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Lep leptin [ Mus musculus ]
• Official Symbol: Lep
• Synonyms: Lep; Leptin; ob; obese
• Gene ID: 16846
• mRNA Refseq: NM_008493
• Protein Refseq: NP_032519
• UniProt ID: P41160
• Chromosome Location: 6 A3.3; 6 10.5 cM
• Pathway: Adipocytokine signaling pathway; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction
• Function: growth factor activity; hormone activity; protein binding