Recombinant Mouse Lag3 protein, His-tagged

• Cat.No.: Lag3-1083M
• Product Overview: Recombinant Mouse Lag3 protein(Q61790)(Ser23-Leu442), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Ser23-Leu442
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse LAG3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse FGL1, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Lag3 lymphocyte-activation gene 3 [ Mus musculus ]
• Official Symbol: Lag3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; Ly66; CD223; LAG-3
• Gene ID: 16768
• mRNA Refseq: NM_008479
• Protein Refseq: NP_032505