Recombinant Mouse Klk8 protein, His & GST-tagged

• Cat.No.: Klk8-798M
• Product Overview: Recombinant Mouse Klk8 aa. (Ile33~Thr250) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Ile33~Thr250
• Description: This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. Mice lacking the encoded protein exhibit impaired long-term potentiation and increased anxiety, as well as a hyperkeratosis phenotype. This gene is located in a cluster of several related kallikrein genes on chromosome 7.
• Form: Freeze-dried powder
• Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Klk8 kallikrein related-peptidase 8 [ Mus musculus (house mouse) ]
• Official Symbol: Klk8
• Synonyms: NP; BSP1; Nrpn; Prss19; Brain Serine protease 1; protease, serine, 19 (neuropsin); kallikrein-8
• Gene ID: 259277
• mRNA Refseq: NM_001324398.1
• Protein Refseq: NP_001311327.1
• UniProt ID: Q61955