Recombinant Mouse Jak3 Protein, Myc/DDK-tagged

• Cat.No.: Jak3-3628M
• Product Overview: Purified recombinant protein of mouse full-length Janus kinase 3 (Jak3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 122.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Jak3 Janus kinase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Jak3
• Synonyms: JAK3; Janus kinase 3; tyrosine-protein kinase JAK3; fae; wil
• Gene ID: 16453
• mRNA Refseq: NM_010589
• Protein Refseq: NP_034719
• UniProt ID: Q62137