Recombinant Mouse Htra1 Protein, Myc/DDK-tagged

• Cat.No.: Htra1-3462M
• Product Overview: Purified recombinant protein of mouse full-length HtrA serine peptidase 1 (Htra1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
• Molecular Mass: 51.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Htra1 HtrA serine peptidase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Htra1
• Synonyms: HTRA1; HtrA serine peptidase 1; serine protease HTRA1; serine protease 11; protease, serine, 11 (Igf binding); high-temperature requirement A serine peptidase 1; insulin-like growth factor binding protein 5 protease; L56; HTRA; Prss11; RSPP11; AI429470
• Gene ID: 56213
• mRNA Refseq: NM_019564
• Protein Refseq: NP_062510
• UniProt ID: Q9R118