Recombinant Mouse Henmt1 Protein, Myc/DDK-tagged

• Cat.No.: Henmt1-3383M
• Product Overview: Purified recombinant protein of mouse full-length HEN1 methyltransferase homolog 1 (Arabidopsis) (Henmt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
• Molecular Mass: 45.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Henmt1 HEN1 methyltransferase homolog 1 (Arabidopsis) [ Mus musculus (house mouse) ]
• Official Symbol: Henmt1
• Synonyms: Henmt1; HEN1 methyltransferase homolog 1 (Arabidopsis); Hen; Hen1; mHEN1; 4921515J06Rik; small RNA 2'-O-methyltransferase; EC 2.1.1.n8
• Gene ID: 66715
• mRNA Refseq: NM_025723
• Protein Refseq: NP_079999
• UniProt ID: Q8CAE2