Recombinant Mouse Hc Protein, His-tagged

• Cat.No.: Hc-375M
• Product Overview: Recombinant Mouse Hc Protein (Asn679-Arg755) with N-His tag was expressed in 293F.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Asn679-Arg755
• Description: This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 10.5 kDa
  Accurate Molecular Mass: 14 kDa
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Hc hemolytic complement [ Mus musculus (house mouse) ]
• Official Symbol: Hc
• Synonyms: Hc; hemolytic complement; C5; He; C5a; Hfib2; complement C5; hepatic fibrogenesis 2
• Gene ID: 15139
• mRNA Refseq: NM_010406
• Protein Refseq: NP_034536
• UniProt ID: P06684