Recombinant Mouse Grk5 Protein, MYC/DDK-tagged
Cat.No. : | Grk5-1051M |
Product Overview : | Purified recombinant protein of full-length mouse G protein-coupled receptor kinase 5 (Grk5), with C-terminal MYC/DDK tag, was expressed in HEK293T cells. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). |
Molecular Mass : | 67.7 kDa |
Purity : | >80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 µg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Grk5 G protein-coupled receptor kinase 5 [ Mus musculus (house mouse) ] |
Official Symbol | Grk5 |
Synonyms | Grk5; G protein-coupled receptor kinase 5; Gprk5; G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5; EC 2.7.11.16 |
Gene ID | 14773 |
mRNA Refseq | NM_018869 |
Protein Refseq | NP_061357 |
UniProt ID | Q8VEB1 |
◆ Recombinant Proteins | ||
GRK5-2359H | Recombinant Human GRK5 Protein, MYC/DDK-tagged | +Inquiry |
GRK5-13541H | Recombinant Human GRK5, His-tagged | +Inquiry |
GRK5-1735H | Recombinant Human GRK5 Protein, Myc/DDK-tagged, C13 and N15-labeled | +Inquiry |
GRK5-2366R | Recombinant Rat GRK5 Protein, His (Fc)-Avi-tagged | +Inquiry |
Grk5-1051M | Recombinant Mouse Grk5 Protein, MYC/DDK-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
GRK5-640HCL | Recombinant Human GRK5 cell lysate | +Inquiry |
Not For Human Consumption!
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