Recombinant Mouse Fzr1 Protein, Myc/DDK-tagged

• Cat.No.: Fzr1-3120M
• Product Overview: Purified recombinant protein of mouse full-length fizzy and cell division cycle 20 related 1 (Fzr1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 55.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fzr1 fizzy and cell division cycle 20 related 1 [ Mus musculus (house mouse) ]
• Official Symbol: Fzr1
• Synonyms: FZR1; fizzy/cell division cycle 20 related 1 (Drosophila); fizzy-related protein homolog; cdh1/Hct1 homolog; cell division cycle 20 related 1; FZR; Fyr; Cdh1; FZR2; HCDH; HCDH1; AW108046
• Gene ID: 56371
• mRNA Refseq: NM_019757
• Protein Refseq: NP_062731
• UniProt ID: Q9R1K5