Recombinant Mouse Furin protein, His-tagged

• Cat.No.: Furin-7821M
• Product Overview: Recombinant Mouse Furin aa. (Arg418~Thr573 (Accession # P23188)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg418~Thr573
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Furin furin (paired basic amino acid cleaving enzyme) [ Mus musculus (house mouse) ]
• Official Symbol: Furin
• Synonyms: C8a; complement component 8, alpha polypeptide; complement component C8 alpha chain; complement component 8 alpha subunit
• Gene ID: 18550
• mRNA Refseq: NM_001081454.2
• Protein Refseq: NP_001074923.1
• UniProt ID: P23188