Recombinant Mouse Foxo1 protein, His-tagged

• Cat.No.: Foxo1-7635M
• Product Overview: Recombinant Mouse Foxo1 aa. (Arg222~Pro469) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Arg222~Pro469
• Form: Freeze-dried powder
• Molecular Mass: 22&25&27&33kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.5.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Foxo1 forkhead box O1 [ Mus musculus (house mouse) ]
• Official Symbol: Foxo1
• Synonyms: Afxh; FKHR; Fkhr1; Foxo1a; AI876417; forkhead box O1a; forkhead box protein O1A; forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead box protein O1
• Gene ID: 56458
• mRNA Refseq: NM_019739.3
• Protein Refseq: NP_062713.2
• UniProt ID: Q9R1E0