Active Recombinant Mouse FOLR1 Protein, His & Avi-tagged, Biotinylated

• Cat.No.: FOLR1-317M
• Product Overview: Recombinant Mouse FOLR1 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Thr 25 - Ser 232 (Accession # P35846-1). Predicted N-terminus: Thr 25. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 26.9 kDa. The protein migrates as 36 kDa and 44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Thr 25 - Ser 232
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Folic acid-BSA at 5 μg/mL (100 μL/well) can bind Recombinant Mouse FOLR1 Protein, His & Avi-tagged, Biotinylated with a linear range of 0.008-0.222 μg/mL (QC tested).
• Molecular Mass: 26.9 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Folr1 folate receptor 1 (adult) [ Mus musculus ]
• Official Symbol: FOLR1
• Synonyms: FOLR1; folate receptor alpha; FR-alpha; folate binding protein 1; FBP1; Folbp1; Folbp-1
• Gene ID: 14275
• mRNA Refseq: NM_001252552
• Protein Refseq: NP_001239481