Recombinant Mouse Fibroblast Growth Factor 2

• Cat.No.: Fgf2-664M
• Product Overview: Recombinantmouse Fgf2 is a disulfide-linked homodimeric protein consisting of 146 aminoacid residue subunits, and migrates as an approximately 16 kDa protein undernon-reducing and reducing conditions in SDS-PAGE, and was lyophilized from0.2 μm filtered PBS solution, pH 7.0.

Specification

• Species: Mouse
• Source: Mouse
• Tag: Non
• Description: basic Fgf2 is foundin almost all tissues of mesodermal and neuroectodermal origin and also intumors derived from these tissues. Endothelial cells produce large amounts ofthis factor. Some Fgf2 is associated with the extracellular matrix of thesubendothelial cells. Many cells express Fgf2 only transiently and store itin a biologically inactive form. The mechanism by which the factor isreleased by the cells is not known. It is released after tissue injuries andduring inflammatory processes.
• Purity: >98%, asdetermined by SDS-PAGE and HPLC.
• Sequence: MAASGITSLP ALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
• Molecular Weight: Native MouseFgf2 basic is generated by the proteolytic removal of the signal peptideand propeptide,the molecule has a calulated mass of 16 kDa.
• Biological Activity: The ED(50) wasdetermined by the dose-dependent stimulation of thymidine uptake by BaF3cells expressing Fgf2 receptors is ≤ 0.2 ng/ml, corresponding to a specificactivity of ≥ 1 x 106 units/mg.
• Endotoxin: Endotoxin contentwas assayed using a LAL gel clot method. Endotoxin level was found to be lessthan 0.1 ng/µg(1EU/µg).
• Reconstitution: A quick spin of thevial followed by reconstitution in distilled water to a concentration notless than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Storage: The lyophilized protein is stable forat least 2 years from date of receipt at -20°C. Upon reconstitution, thiscytokine can be stored in working aliquots at 2°C - 8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss ofactivity. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: Fgf2
• Pathways: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; Endochondral Ossification; FGFRligand binding and activation; FGFR1 ligand binding and activation; FGFR1bligand binding and activation; FGFR1c ligand binding and activation; FGFR2ligand binding and activation; FGFR2c ligand binding and activation; FGFR3ligand binding and activation; FGFR3c ligand binding and activation; FGFR4ligand binding and activation; Melanoma; PI-3K cascade; PI-3K cascade

Gene Information

• Gene Name: Fgf2 fibroblast growth factor 2 [ Mus musculus ]
• Synonyms: Fgf2; fibroblastgrowth factor 2; Fgfb; bFGF; Fgf-2; heparin-binding growth factor 2; HBGF-2;OTTMUSP00000046776; basic fibroblast growth factor
• Gene ID: 14173
• mRNA Refseq: NM_008006
• Protein Refseq: NP_032032
• UniProt ID: P15655
• Function: chemoattractantactivity; cytokine activity; fibroblast growth factor binding; fibroblastgrowth factor receptor binding; growth factor activity; heparin binding; ligand-dependentnuclear receptor transcription coactivator activity; protein binding; voltage-gatedcalcium channel activity