Recombinant Mouse Fhit Protein, Myc/DDK-tagged

• Cat.No.: Fhit-3010M
• Product Overview: Purified recombinant protein of mouse full-length fragile histidine triad gene (Fhit), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a member of the HIT family of proteins that are characterized by the presence of a histidine triad sequence. The encoded protein is a diadenosine triphosphate hydrolase enzyme that cleaves the P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. This locus is very fragile and has been found to be altered in different types of cancers. Mice lacking the encoded protein display increased susceptibility to spontaneous and induced tumors. Ectopic expression of the encoded protein in such knockout mice inhibits tumor development. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Molecular Mass: 17.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fhit fragile histidine triad gene [ Mus musculus (house mouse) ]
• Official Symbol: Fhit
• Synonyms: FHIT; fragile histidine triad gene; bis(5-adenosyl)-triphosphatase; AP3Aase; AP3A hydrolase; dinucleosidetriphosphatase; fragile histidine triad protein; diadenosine triphosphate hydrolase; diadenosine 5,5-P1,P3-triphosphate hydrolase; Fra14A2; AW045638
• Gene ID: 14198
• mRNA Refseq: NM_010210
• Protein Refseq: NP_034340
• UniProt ID: O89106