Recombinant Mouse Fbxl2 Protein, Myc/DDK-tagged

• Cat.No.: Fbxl2-2962M
• Product Overview: Purified recombinant protein of mouse full-length F-box and leucine-rich repeat protein 2 (Fbxl2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G0. SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy. PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 46.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fbxl2 F-box and leucine-rich repeat protein 2 [ Mus musculus (house mouse) ]
• Official Symbol: Fbxl2
• Synonyms: FBXL2; F-box and leucine-rich repeat protein 2; F-box/LRR-repeat protein 2; Fbl3; 2810423A21Rik
• Gene ID: 72179
• mRNA Refseq: NM_178624
• Protein Refseq: NP_848739
• UniProt ID: www.uniprot.org/uniprot/Q8BH16