Recombinant Mouse Exo5 Protein, Myc/DDK-tagged

• Cat.No.: Exo5-2885M
• Product Overview: Purified recombinant protein of mouse full-length exonuclease 5 (Exo5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-ends. Acts as a sliding exonuclease that loads at ssDNA ends and then slides along the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 41.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Exo5 exonuclease 5 [ Mus musculus (house mouse) ]
• Official Symbol: Exo5
• Synonyms: Exo5; exonuclease 5; Dem1; Exo V; mExo5; AV297100; 3110037I16Rik; exonuclease V; defects in morphology 1 homolog; defects in morphology protein 1 homolog; probable exonuclease V
• Gene ID: 73172
• mRNA Refseq: NM_001160043
• Protein Refseq: NP_001153515
• UniProt ID: Q9CXP9