Recombinant Mouse Epcam Protein, His-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Epcam-283MAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant Mouse Epcam (Gln24-Thr266) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.
  Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 28.5 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln24-Thr266
• N-terminal Sequence Analysis: Gln 24
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Epcam
• Official Symbol: Epcam
• Synonyms: EPCAM; epithelial cell adhesion molecule; mEGP314; protein 289A; Trop-1 protein; lymphocyte antigen 74; epithelial glycoprotein 314; panepithelial glycoprotein 314; tumor-associated calcium signal transducer 1; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; Tacsd1; GA733-2; Tacstd1
• Gene ID: 17075
• mRNA Refseq: NM_008532
• Protein Refseq: NP_032558
• UniProt ID: Q99JW5