Recombinant Mouse Eif4a1 Protein, Myc/DDK-tagged

• Cat.No.: Eif4a1-2784M
• Product Overview: Purified recombinant protein of mouse full-length eukaryotic translation initiation factor 4A1 (Eif4a1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
• Molecular Mass: 46.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Eif4a1 eukaryotic translation initiation factor 4A1 [ Mus musculus (house mouse) ]
• Official Symbol: Eif4a1
• Synonyms: EIF4A1; eukaryotic translation initiation factor 4A1; eukaryotic initiation factor 4A-I; eIF4A-I; eIF-4A-I; ATP-dependent RNA helicase eIF4A-1; initiation factor eIF-4A long form; Eif4; Ddx2a; BM-010
• Gene ID: 13681
• mRNA Refseq: NM_144958
• Protein Refseq: NP_659207
• UniProt ID: P60843