Recombinant Mouse Ecd Protein, Myc/DDK-tagged

• Cat.No.: Ecd-2714M
• Product Overview: Purified recombinant protein of mouse full-length ecdysoneless cell cycle regulator (Ecd), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 71.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ecd ecdysoneless cell cycle regulator [ Mus musculus (house mouse) ]
• Official Symbol: Ecd
• Synonyms: ECD; ecdysoneless homolog (Drosophila); protein SGT1 homolog; suppressor of S. cerevisiae gcr2; Hsgt1; 5730461K03Rik
• Gene ID: 70601
• mRNA Refseq: NM_027475
• Protein Refseq: NP_081751
• UniProt ID: Q9CS74